Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol

Abstract Background ( R )-(+)-perillyl alcohol is a naturally oxygenated monoterpene widely used as the natural flavor additives, insecticides, jet fuels and anti-cancer therapies. It was also readily available precursors. However, this product present at low concentrations from plant sources which are not economically viable. Therefore, alternative microbial production methods rapidly emerging an attractive to make more sustainable environmentally friendly. Results We engineered Escherichia coli possess heterologous mevalonate (MVA) pathway, including limonene synthase , P-cymene monoxygenase hydroxylase reductase for of alcohol. The concentration )-(+)-limonene (the precursor alcohol) reached 45 mg/L glucose. Enhanced therefore achieved. strain produced titer 87 yield 1.5 mg/g glucose in 5 L bioreactor fed batch system. Conclusions These datas highlight efficient through pathway This method serves platform future other monoterpenes.